Cleaning Procedure For Biological Safety Cabinet

Spray the surfaces of all items placed into the cabinet with 70 ethanol to minimize the introduction of contaminants into the bsc.

Cleaning procedure for biological safety cabinet.

Aseptic technique and daily decontamination will eliminate the majority of contaminants. 5 1 2 dedust the biosafety cabinet daily externally with a clean dry cloth. Once the cabinet has been operational for 15 minutes disinfect all interior surfaces of the cabinet thoroughly by spraying with 70 ethanol. To begin apply disinfect to a towel and decontaminate and remove items from the area of the spill.

Remember to wear appropriate ppe and leave the biosafety cabinet on during spill cleanup. μ microns in diameter with higher efficiency for both larger and. Allow to air dry. 5 1 3 once in a week remove adhered dust by wet mopping using soap solution.

How to clean a biological safety cabinet lafw hood. Hepa stands for high efficiency particulate air. If the biological safety cabinet is turned off between aseptic processes for routine maintenance or any other reason it should be operated long enough to allow complete purging of room air from the critical area for at least 30 minutes then cleaned and disinfected before use. Hepa filters are defined as air cleaning devices that have a proven minimum removal efficiency of 99 97 of particles in the air equal to 0 3.

Cleaning a biosafety cabinet or bsc is an important function in terms of both containment and sterility. Biological safety cabinet sop bsc 01. Once the cabinet is empty you should allow 20 30 minutes to thoroughly decontaminate all interior work surfaces making sure to clean the back and side of the cabinet and the interior of the glass. Put the waste from the spill into a biohazard bag inside the cabinet.

How to clean a biological safety cabinet lafw hood. This may take more or less time depending on the disinfectant and the microbiological agent used. 10 easy steps to cleaning a contaminated surface in the biosafety cabinet. The biohazard bag should never be kept outside the cabinet.

Cleaning and disinfection 1. 5 0 procedure 5 1 general cleaning procedure 5 1 1 ensure that the power supply of the biosafety cabinet is switched off. The biological safety officer will determine the type of decontamination necessary. Afterward wipe the surface with a clean dry cloth to remove the moisture.

The interior surfaces should also be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. When work is completed all equipment and supplies from the bsc should be decontaminated and removed from the cabinet.